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Mouse kidneys were transfected with an adeno-associated virus vector (AAV) Eight-week-old mice received in situ kidney injection with adeno-associated virus vector (AAV)

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Crocin can inhibit the expression of tight junction proteins, enhance the loss of nicotinamide adenine dinucleotide phosphate oxidase, block the induction of MMP-2 and MMP-9 by cerebral ischemia, thereby exerting antioxidant and BBB integrity-maintaining effects (Zhang et al., 2017)