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Detection of DNA damage by TAM and its metabolites in the presence of NADH and Cu(II) A standard reaction mixture (in a 1.5 ml Eppendorf microtube) contained the 3,4-diOH-TAM, CuCl 2 , NADH, 32 P -5-end labeled DNA fragments and calf thymus DNA (510 M per base) in 200 l of 10 mM sodium phosphate buffer (pH 7.8) containing 5 M DTPA

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