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10.1007/s00228-012-1410-7 30 JamesL

Participants with other diseases which could influence biological indicators were excluded, such as diabetes mellitus, hypertension, hyperthyroidism, hyperlipidemia, alcohol abuse, rheumatoid arthritis, or tumor

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Additionally, fresh blood was collected from the mice after treatment and centrifuged at 1500 g for 15 min to obtain serum supernatant, and levels of TNF- and IL-6 in the serum were measured using ELISA kits, following the manufacturers protocols

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doi: 10.4239/wjd.v6.i1.80 97 BrowningKNVerheijdenSBoeckxstaensGE