[DOI] [PubMed] [Google Scholar] 413.Matsunaga T., Shoji A., Gu N., Joo E., Li S., Adachi T., Yamazaki H., Yasuda K., Kondoh T., Tsuda K
aCSF was removed by careful suctioning and replaced with aCSF containing vehicle (aCSF+aCSF), 100 ng/ml with carrier free adult recombinant Hepatocyte Growth Factor (HGF) (R and D Systems, MN, USA) in aCSF, 10 10 M Hinge (Harding lab), 50 ng/ml in aCSF, 10 10 M Dihexa (Harding lab) in aCSF, 10 12 M Dihexa in aCSF or 50 ng/ml HGF+10 12 M Dihexa in aCSF for 30 minutes at 37 C
Severe BIPN can result in muscle atrophy, diminished ankle reflexes, and reduced proprioception, leading to foot drop, ataxia, and gait disturbances, all of which can impair daily functioning (Yamamoto and Egashira, 2021
The supernatant was collected, and the protein concentration was determined with a BCA protein assay kit (Cat
Sousa-Figueiredo, J
An entire infusion session typically takes 45 to 60 minutes, and there is no downtime afterward