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for 3 h and then concentrated under vacuum

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The spermidine synthase (EC 2.5.1.16) mediated addition of aminopropyl from decarboxylated s-adenosylmethionine led to the synthesis of triamine spermidine and, finally, spermine synthase (EC 2.5.1.22) transfers an additional aminopropyl group to form the tetramine spermine [126]

Giralt, M., Lafuente, A., Pujol, F