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Reconstitution must be performed by trained personnel in an appropriate environment (e.g., a laminar flow cabinet or isolator where required by the protocol)

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In the enrichment sub-cultures, the communitys nutritional requirements were met by either compounds dissolved in the medium itself (Table 1) biomass or byproducts of other community members [e.g., exuded organic carbon (Christie-Oleza et al., 2017