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The damage from freeze-thaw cycles comes from repeated freezing and thawing of the same solution, where ice crystals form, melt, reform, and melt again, each cycle physically stressing the peptide molecules

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DECLARATIONS Authors contributions Developed the model, ran simulations and analyses, interpreted the findings, and contributed to the writing and editing of the manuscript: Cruikshank A, Reed MC, Nijhout HF Availability of data and materials Computer models and codes used in this paper can be obtained from the authors by request

It should be kept out of reach of children

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