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283 Mitochondria in aging and oxidative stress Harman proposed the free radical theory of aging in the 1950s, which hypothesized that the increase of free radicals, especially oxygen free radicals, would induce oxidative stress, causing damage to a variety of bioactive substances and accelerating aging

2.4 Determination of fecal water content Fresh feces of rats were weighed and dried in the oven to a constant weight with the temperature set at 105C