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For deglycosylation assay from the membrane fraction, the pelleted cells were resuspended in 1 ml ice-cold membrane isolation buffer (1 mM EDTA and 1 mM EGTA in 1X PBS, with protease inhibitor) and homogenized

T., Kurenda, A., Stolz, S., Chetelat, A

Each treatment takes about 30-60 minutes, making it a great option for people with busy schedules

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