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Free-amino-acid samples were derived using derivatizing solution (ethanol:distilled H 2 O:triethylamine:phenylisothiocyanate at 7:1:1:1, v/v/v/v) for 15 min

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At the same time, the remaining part of the liver was soaked in a 10% buffer formalin solution and stored at -20C for later histopathological examination

Results from a two-sample Mendelian randomization study demonstrate a causal relationship between Akkermansia and hypothyroidism, indicating that Akkermansia may inhibit the occurrence and progression of hypothyroidism (Shi et al., 2024