Cultured human umbilical vein ECs (HUVECs), in particular, express typical molecular markers, including CD31, von Willebrand factor (VWF), CD34, intercellular adhesion molecule-1 (ICAM-1), E-selectin, vascular cell adhesion molecule-1 (VCAM-1), and angiotensin I converting enzyme (ACE) [15, 16] (Table 1)
The reaction was initiated by the addition of 190 l reaction buffer, adjusted to pH 7.4 by titration with K 2 HPO 4 (40 mM) and KH 2 PO 4 (10 mM) stock solutions, supplemented with NADH (100 M) and sodium pyruvate (600 M)
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G-yS: Conceptualization, Funding acquisition, Writing original draft, Writing review & editing, Project administration, Data curation
serum and plasma [5760], synovial and uterine fluids [59, 61], intestinal Paneth cells [62, 63] and granulocytes [31] (Figure 1)
