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Yolland CO, Phillipou A, Castle DJ, Neill E, Hughes ME, Galletly C, et al

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For this, the organs were aseptically removed and homogenized in 1 ml of H2O using a GentleMacs dissociator (Miltenyi Biotec)

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Obesity-associated microbiomes instigate visceral adipose tissue inflammation by recruitment of distinct neutrophils

The gene expression level was normalized to the level of GAPDH (Forward: 5-ACCCACTCCTCCACCTTTGA-3 and Reverse 5-TGTTGCTGTAGCCAAATTCGTT-3) and determined using the C T method