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(1530 mL) mixed with water and taken before or after meals

The classic warning sign is persistent, severe abdominal pain that may spread to the back, sometimes with nausea or vomiting

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Outer thigh and back of upper arm are alternative sites

ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads, by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, United States) connected to a Waverunner LT342 digital oscilloscope (LeCroy, United States) at 30 min ligation time