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When applicable, cells were transfected 24 h later as described above

In the Purkinje cell layer, the mean number of dark neurons significantly increased in the CN group (2.465 0.135/10 m 2 ) in comparison with the VC group (P = 0.0049, Figures 4C, D, I)

The role of autophagy is particularly context-dependent

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If no, go to step iv