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Analysis by HPLC with fluorescence detection was performed as previously described 43,44

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A single oral dose of 45 mg/kg panduratin A-consisted fingerroot extract showed a significantly lower AUC than a single oral dose of 45 mg/kg pure panduratin A, with absolute oral bioavailability of approximately 6 and 9%, respectively

During the treatment process, nanomedicine (5 mg/kg) was injected into the tail vein of mice on days 0 and 5, respectively, and US irradiation (10 min, 1.5 W/cm 2 , 1 MHz, 50% duty cycle) was performed on days 1 and 6

Freezing should be avoided as ice crystal formation can damage the peptide structure

The effective way to reduce side effects is to increase the dose slowly