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Meanwhile, organoids were collected according to the above protocol, except that after the initial centrifugation step, the organoids were resuspended in 3 mL TrypLE Express containing 10 M Y-27632 and incubated in a 37 C water bath for 10 min, followed by trituration 10 times with a P1000 pipette followed by a P10 tip attached to the P1000 tip

doi: 10.1002/jcla.20325 511 KonstantinovKvon MikeczABuchwaldDJonesJGeraceLTanEM

Improving mitochondrial lipotoxicity defense The regulation of lipid droplet-mitochondrial interactions deserves attention

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This work was supported by the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Young Scientists (B) Grant Number JP15K19036 (SH), JSPS Grant-in-Aid for Young Scientists Grant Number JP19K16271 (SH), Japan Science and Technology Agency (JST) ACT-X Grant Number JPMJAX201A (SH), RIKEN FY2018 Incentive Research Projects (SH), and Naito Grant for female scientists after maternity leave (SH)