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3A) and controls (Fig

The BBB and BCSFB are formed by tight junctions of brain capillary endothelial cells and choroid plexus epithelial cells, respectively, and serve to regulate the supply of essential nutrients into the brain/CSF and the elimination of xenobiotics and endogenous metabolites from the brain/CSF via various transport systems such as transporters [5, 6]

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Remove air bubbles from the syringe by holding it needle-up and tapping gently, then pushing the plunger slightly to expel trapped air