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Methods Animals We used 6-week-old male C57BL/6J mice (obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology) and 6-week-old male Nrf2 knockout mice (B6.129 1-Nfe2l2 tm1Ywk /J, purchased from Jackson Laboratory, Bar Harbor, ME) for the experiments

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A peptide credited with somnogenic, analgesic, anti-stress, endocrine, and anti-withdrawal actions, with no receptor and no established pathway linking any of them, is a peptide whose reported activity has never been localized

Understanding how they might work together is where real practical value emerges

Dose translation from animal to human studies revisited