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Relationship between RS, Aging, and Related Diseases Reactive oxygen, nitrogen, and halogen species, collectively referred to as reactive species (RS), are produced through various internal and external processes

Their presence in supplements supports their use in general dietary formulations

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The cDNA was then fourfold diluted and used as a template in quantitative PCR with IQ SYBR Green Supermix (Bio-Rad) on a CFX96 instrument (Bio-Rad) using a 3-step protocol