Methods Strains, plasmids and molecular biology methods All the strains and plasmids used in this study are listed in Table 1
Furthermore, elevated cytosolic aconitase (c-ACNT) and isocitrate dehydrogenase (ICD2), synergistically enhanced by iron accumulation, drive citrate flux toward NADPH and -KG generation, thereby exacerbating intracellular citrate depletion
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a RLM (1D): RLM incubation without fractionation, RLM (2D): RLM incubation with fractionation, 3A4 (1D): CYP3A4 Supersome incubation without fractionation, 3A4 (2D): CYP3A4 Supersome incubation with fractionation, DDA: confirmed by DDA, MRM: confirmed by MRM