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The suggested dithiol mechanism was based on experiments at a single substrate concentration that required more than 8 h for redox equilibration in the presence of 0.5 M glutaredoxin with or without 1 M GSH

1) Dose , 2) Duration & Frequency , 3) Individual susceptibility (genetics, nutrition, stress, hydration, sleep)

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With excellent biocompatibility, TPE-DCV can also be used for mapping the distribution of intracellular free thiols, mainly GSH

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