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PVDF membranes (Millipore, IPFL07810) were activated in methanol for 30 s and rinsed in transfer buffer (Invitrogen, NP00061)
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doi: 10.3389/fimmu.2020.593103 232 Garcia-GonzalezAGonzalez-LopezLValera-GonzalezICCardona-MuozEGSalazar-ParamoMGonzlez-OrtizMet al
Creating topical GHK-Cu serum 1% GHK-Cu serum (common strength): 50mg GHK-Cu powder Mix with 5g (5ml) carrier (hyaluronic acid serum, gel) Creates 1% concentration Apply daily to face 2% GHK-Cu serum (stronger): 50mg GHK-Cu powder Mix with 2.5g carrier Creates 2% concentration More powerful but can irritate Carrier options: Hyaluronic acid serum (best) Aloe vera gel Water-based serum Niacinamide serum How to make: Purchase quality carrier serum Add GHK-Cu powder to serum Mix thoroughly until dissolved Store in dark bottle Refrigerate Use within 30 days Topical application protocol Daily application: Cleanse face thoroughly Apply 2-3 drops serum to face and neck Massage gently until absorbed Morning and/or evening Follow with moisturizer if desired Where to apply: Entire face Neck and dcolletage Around eyes (careful, avoid in eyes) Forehead wrinkles Any area with aging concerns Expected results: Week 4-6: Skin texture improving Week 8-10: Visible firmness increase Week 12+: Wrinkle reduction, tone improvement Combining with skincare: Use GHK-Cu before moisturizer Can combine with retinol (see timing below) Compatible with vitamin C, niacinamide Use SPF during day (always) See our can you use peptides and retinol together , hyaluronic acid peptide , and copper peptides ruined my skin guide
This age-related NAD+ depletion has prompted interest in NAD+ supplementation strategies to support cellular metabolism and possibly counteract aspects of aging
