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Notably, in the case of the modification, the binding constant was only 1.4 times higher

2 Dual-column LC-HRMS data acquisition Each sample is run in two separate LC-HRMS acquisitions: reversed-phase (RP) chromatography for non-polar and moderately polar metabolites, and hydrophilic interaction liquid chromatography (HILIC) for highly polar species (amino acids, nucleotides, organic acids, sugars)

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10.1155/2022/1767421 483 WuM.ChengY.ZhangR.HanW.JiangH.BiC.et al