It is well-recognized that the proportion of complex carbohydrates or fiber (typically found in the diet as long-stem forages) to more simple carbohydrates (such as starches and sugars), as well as abrupt changes in diet, cause fluctuations in the populations of microbes within the hindgut of the horse [109,122124]
Group 1 was a vehicle control that received an intravenous injection of saline
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Positive (virus-infected cell culture) and negative (water) controls were included from the beginning of the extraction procedure until the reading of the results