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This is usually a gradual, subtle shift rather than a dramatic change

Levels of additional cytokines in cell culture supernatants from Fig

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During a session, a chelating agent is administered either intravenously or orally

Assays contained 0.25 nM human recombinant IDH1 (RD systems), 30 M isocitrate, and 30 M NADP + in 20 L of 100 mM Tris-HCL, pH8, 0.2 mM DTT, 0.05% CHAPS, and the indicated MgCl2 levels