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Apr 2014;69:403-416

The results depend on personal skin characteristics and metabolic rate and the chosen treatment approach and how well you follow skincare advice

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The resulting cDNA was used as a template for quantitative RT-PCR, which was performed with primers specific for TGF-1 and -actin (see in Table 1 ), according to the instructions for the SYBR Premix EX Taq Kit (TaKaRa Biotechnology Co., Ltd.), and a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, United States)