The corresponding test reports, including verification codes, are publicly available
doi: 10.3390/molecules201219800 101
Mao R, Zong N, Hu Y, Chen Y, Xu Y
More particularly, for each baseline (and progression, respectively) sample, SAMtools version 1.9 mpileup 49 was run on the positions where Mutect2 identified and retained mutations in the corresponding progression (and baseline, respectively) sample to rule out incorrect claims of mutation acquisition or loss caused by conservative filtering or non-detection by Mutect2
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Step 5: Hold and remove After the plunger is fully depressed, count to five before removing the needle