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Tubulin expression was detected on the same PVDF membrane using anti--tubulin specific monoclonal antibody (1 g/ml Sigma-Aldrich, Ont., CA), followed by HRP-conjugated goat anti-mouse IgG (1:5000 (v/v), BioRad, Ont., CA)

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When DNA damage is reparable, these DNA damage foci are temporary

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