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Cell Counting kit (CCK-8) viability and cell death assay Cells were seeded into 96-well plates at a density of 3 10 cells per well and subjected to the designated treatments for 72 h

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Hematologic and biochemical monitoring throughout the trials revealed no concerning patterns in blood counts, liver function tests, kidney function markers, or other laboratory parameters

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Despite randomized clinical trials demonstrating that amlodipine did not exhibit favorable clinical effects for the treatment of chronic heart failure (45, 46), amlodipine therapy could be used as adjunctive therapy in cases of iron overload, as seen in our patient's case and recent randomized clinical trials of amlodipine in patients with iron overload ( 5