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Cells in the logarithmic growth phase were harvested from culture flasks and adjusted to a density of 6.0 10 3 cells/well in 96-well plates (100 L/well), followed by incubation at 37 for 24 h

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Pflugers Arch 442:28696 Sato H, Shiiya A, Kimata M, Maebara K, Tamba M, Sakakura Y, Makino N, Sugiyama F, Yagami K, Moriguchi T, Takahashi S, Bannai S (2005) Redox imbalance in cystine/glutamate transporter-deficient mice