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Through Western Blot detection results, the corresponding bands of GST-X protein and Y protein can be seen, indicating that X protein and Y protein are pulled down by GST-X due to their interaction
Oxidation of methionine, deamidation (N, Q) and acetylation (protein N-terminus) as optional modification, carbamidomethylation (C) as a fixed modification and trypsin/P enzyme with two allowed miss cleavages were set
Cell Rep Med (2023) 4(11):101254
[20] [7] This conversion is illustrated by the reduction of peroxides (via the sulfenate as an intermediate): GSH + R 2 O 2 GSOR + ROH GSOR + GSH GSSG + ROH Net reaction: 2 GSH + R 2 O 2 GSSG + 2 ROH (R = H, alkyl) and with free radicals, forming glutathionyl radicals (GS ) that may dimerize to disulfide: GSH + R RH + GS 1/2 GSSG Thiyl radicals (such as glutathionyl radicals) are themselves oxidizing species in biology, with a single-electron reduction potential sufficient for oxidation of nucleic acids, proteins and polyunsaturated lipids
This sensitivity isnt unique either
Mobasheri, A
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