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This peptide was first isolated and identified in the 1990s by Croatian researchers, who observed its remarkable protective properties on gastrointestinal tract tissues

GHK-cu also exhibits antioxidant and anti-inflammatory actions, making it a versatile compound for skin biology, tissue regeneration, and anti-aging studies

[9] [5] It is given by mouth, by injection into a muscle, or as a nasal spray

For immunohistochemistry, detection was performed using a biotinylated anti-rabbit IgG secondary antibody and streptavidin-Horseradish peroxidase (HRP), followed by colorimetric detection using DAB

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In practice, this becomes more important if weight change would affect blood sugar, self-image, or willingness to stay on the medication