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These changes can be subtle and may not be obvious without careful observation

PMID: 10469335[PubMed Reference] Philp D, Badamchian M, Scheremeta B, et al

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The precipitate is then dialyzed against PBS (pH 7.2) and triphosphate buffer (pH 8.2), followed by further purification and analysis using gel electrophoresis and chromatography