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Immunofluorescence analysis of phosphorylated Met on cells EHMES-1 cells (2 10 4 cells per well) were seeded in a 96-well glass bottom plate and cultured in RPMI1640 medium supplemented with 10% FBS for 24 h

MOTS-c has a 24 hour half-life with effects lasting 1224 hours via AMPK phosphorylation

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Testa G, Mora-Maltas B, Camacho-Barcia L, Granero R, Lucas I, Agera Z, et al