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These issues can be minimized by maintaining the sample at 0C (ice bath) and extracting with metaphosphoric acid as soon as possible

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J Cell Sci 123:20942102 Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A (2006) NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly

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