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After incubation for an appropriate period of time at 37C, the cells were fixed with methanol for 30 min and stained with crystal violet solution for 20 min

Collagen: Assists with elasticity, moisture, and can reduce the appearance of fine lines and wrinkles

VEH treatments were anesthetised with isoflurane and decapitated (n = 58 animals/group)

Astrocytes are particularly important as they are capable of producing large amounts of GSH and cysteine, both of which are exported to support neurons in the CNS

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