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For measuring GPx (EC 1.11.1.9) activity, the mixture had 1 ml of phosphate buffer (50 mM KPO 4 buffer pH 7.0 + 0.1% Triton X-100), 0.1 ml of 2.4 M NADPH, 12 M GSH, 6 U GR and 0.1 ml of hydrogen peroxide

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Lastly, all blood samples were drawn in the morning to control for the potential impact of the circadian rhythm on carnitine levels ( Our findings should be interpreted within the context of a number of limitations