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For example, knockdown of PER1 or CRY2 in mammary epithelial cells impairs cell cycle control and facilitates oncogenic behavior, while mouse models with mutations or deletions in circadian genes develop spontaneous tumors or exhibit heightened sensitivity to mammary carcinogens 200
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Six days post-transfection, the cell culture supernatant was harvested by centrifugation at (290400) g for 25 min at 4 C and filtered using a 0.2-m Vacuum Filtration System (VWR)
For a deeper explanation, see How Long Do Reconstituted Peptides Last When to Use Sterile Water Sterile water is typically preferred in controlled, single-use scenarios