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Next, the spinal surgeon can widen the intervertebral foramen to remove any blockages or causes of compression surrounding a nerve
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Then, cells were incubated with a 1:200 dilutions of primary anti--H2AX antibody (ABclonal Technology, Wuhan, China) overnight at 4 C, followed by three washes with PBS and incubation with FITC-labeled goat anti-rabbit IgG antibody for 1 h and mounted with DAPI [32]
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Lung tissue was processed as follows: one portion was immediately frozen in liquid nitrogen and stored at 80 C for gene expression analysis
