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Reconstituted solution storage requires 2-8C refrigeration with critical light protection (keep in dark or wrapped in foil), in original vial with tight seal, avoiding repeated temperature cycling and never freezing reconstituted solution

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Immunoblotting analysis 80 oocytes were lysed directly in Laemmli sample buffer (1610737, Bio-Rad) containing -mercaptoethanol (M6250, Sigma-Aldrich) and a protease inhibitor cocktail (HY-K0016, MedChemExpress), and were heated at 100 C for 10 min