Immunohistochemistry and stereologic analysis Tissues for histological analysis were fixed with 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4) and then cryoprotected in 30% sucrose overnight at 4C
15 10 ,
Furthermore, owing to different reasons, a considerable number of cancer patients did not respond to these therapeutics efficiently (8,9)
Sandstrom, J., Carlsson, L., Marklund, S.L
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