Efficacy of oral compared with intramuscular vitamin B-12 supplementation after Roux-en-Y gastric bypass: a randomized controlled trial
The cell pellet obtained after Percoll layering was plated in non-precoated culture ware for adherent cells and expanded for 4 days in DMEM/F-12 media with 10% FCS (Sigma, St Louis, MO)
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The secondary endpoints include achievement of 20% weight reduction, relative change in body weight, change in waist circumference, change in HbA1c, change in systolic blood pressure, change in the quality of life (IWQOL-Lite-CT), and change in short-form physical functioning score (SF-36v2)