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Rapid metabolic changes: The speed of weight loss on semaglutide often outpaces the body's ability to adapt

This work was supported by project PCE107/2021 funded by the Ministry of Research, Innovation and Digitalization, CNCS/CCCDIUEFISCDI, within PNCDI III, project PN 19.23.01.01 Smart-Bi and project PN 23.06.02.01 InteGral, Nucleu Programme, funded by Ministry of Research, Innovation and Digitalization

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Enhanced thermogenesis would increase total daily energy expenditure, creating a more favorable energy balance for fat loss

If a reducing-agent-containing assay buffer is required, add GHK-Cu in the final step at the working dilution

Subsequently, 0.1 mM IPTG was added to the transfected cells, which was then cultured with 1 L LB medium for 16 h at 27 C