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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

7 8 NAC's effectiveness as a glutathione precursor does not require high plasma NAC concentrations: once cysteine reaches the target cell, the synthesis machinery uses it efficiently

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TCGA and GEO datasets Transcriptome data of breast cancer (BRCA) cohort from TCGA ( and Gene Expression Omnibus (GEO) dataset (GSE173300, GSE128458, and GSE144378) ( were analyzed

L-Glutathione with CAS 70-18-8 What is L-Glutathione L-Glutathione (GSH) is a naturally occurring tripeptide composed of three amino acids: glutamic acid, cysteine, and glycine

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