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All controlled studies referenced below were conducted in cell culture (3T3-L1 adipocytes, primary muscle stem cells) or rodent models (diet-induced obese mice, aged C57BL/6J mice)

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This balance between size and specificity is frequently cited as a design achievement of the original Monash research program and is one reason the molecule has endured as a reference compound across multiple laboratories

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It means precision matters more outside controlled environments