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Colony replating assay After performing the clonogenic assay as indicated above, cells in the colonies ( n = 3 plates for each condition) were de-attached, and the viable cells were counted, showing similar and representative reductions on absolute viable cell counts (GUAVA easycyte) as previously observed in the number of colonies (Supplementary Fig

Neuropharmacology 55 , 12381250 (2008)

D., Pottenger, L

This could be due to common adaptation strategies employed in extreme environments, such as the accumulation of low molecular weight osmolytes, mainly organic solutes, which are responsible for extreme cold, oxidative stress, starvation and salinity responses (Zhang et al., 2015)

This may indicate differential behavior of these genes in PCa susceptibility, linked to the continental ancestry of each population

Nodules are symmetric or nearly symmetric mid-vocal fold masses