Deficiency can show up as fatigue, weakness, numbness/tingling, balance problems, memory issues, and megaloblastic anemia
To exchange the linkers, maxiprepped libraries of M13-linked scFvs were amplified using Phusion Plus with a pool of overlapping primers annealing to either framework 4 (FW4) of the heavy chain or framework 1 (FW1) of the light chain
Store capsules of B12 at room temperature, not in the fridge
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Safety first: Always use a sterile needle dont touch it