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After 24 h, treated biofilms were washed once with 1 PBS followed by addition of 200 L of 1 PBS and 15 L of a 0.05% w/v resazurin solution

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Physiology-Cell Physiology 290, C1399C1410

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And now it is generally believed that alcohol is harmful to the human gut, leading to a substantial number of IBD patients voluntarily refraining from alcohol consumption [274]

Oxidative stress is commonly discussed in the context of illness and physiologic strain in dogs, and antioxidant supplementation has been proposed as supportive care in those situations (Hagen DM, 2019)