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In fact, using the lowest effective dose is the approach most researchers recommend

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In order to prevent mitochondrial malfunction and sustain metabolic function/energy generation (ATP), NAD+ supplementation may counterbalance the age-related degradation of NAD+ and its precursor nicotinamide mononucleotide by NADases, particularly CD38.7 NAD+ replenishment, however, appears to support a number of other metabolic pathways via NAD+-dependent enzymes in research involving human and animal models (as well as samples and cell lines) (1,2,3)

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After centrifugation (12,000 g , 15 min, 4 C), protein concentrations were determined using a BCA assay (CWBIO, China)