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Changes in the intestinal bacterial community, short-chain fatty acid profile, and intestinal development of preweaned Holstein calves
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The integrity and concentration of the RNA were first assessed by measuring the OD260/OD280 ratio using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific, Inc.) and further confirmed by running samples on 1% agarose gel electrophoresis
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The findings from the investigation also showed a decrease in lipid peroxide level in the -treated group [54]
Furthermore, the persistence of ALB expression despite declining hepatic protein levels supports the notion of functional heterogeneity, potentially reflecting a dynamic interplay between hepatocyte populations and expanding mesenchymal cells
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